Quick Start: Creating Publication-Quality Images with MountainsMap SPM
1. Open and prepare your data
- Load file: File → Open, then select your AFM/STM image (supported formats: .spm, .gwyddion, .txt, common microscope formats).
- Crop: Use the Crop tool to remove damaged edges or scan artifacts.
- Plane/line correction: Apply Plane correction (Polynomial order 1) and Line correction if stripe artifacts remain.
2. Correct artifacts and filter
- Tilt and levelling: Use Leveling → Flatten or Polynomial Fit to remove sample tilt.
- Despeckle/Median filter: Apply a small-radius median filter (1–3 px) to remove isolated spikes without blurring features.
- Low-pass filter: For noise reduction, use a gentle Gaussian low-pass (sigma 0.5–1 px). Avoid over-smoothing.
3. Choose color map and contrast
- Colormap: Select a perceptually uniform colormap (e.g., Viridis, Cividis) to preserve detail and avoid misleading contrasts.
- Scale: Set scale to linear unless your data needs log scaling. Use the histogram or Auto Contrast to set min/max, then fine-tune.
- Clipping: Avoid hard clipping of extrema; instead, use percentile-based limits (e.g., 1–99%) to retain detail.
4. Add annotations and scale
- Scale bar: Insert a scale bar sized to the image (use the known scan size). Place in a corner with contrasting color and thin border.
- Color scale/legend: Add a vertical color scale indicating units (nm, µm).
- Text labels: Use concise labels for channels, magnification, and imaging mode. Choose legible font and size for print.
5. 3D rendering and lighting
- 2D vs 3D: Use 3D hillshade or relief rendering for better topography visualization—keep both 2D and 3D versions for publication.
- Lighting: Set a single light source; adjust azimuth and elevation to emphasize features without creating artificial shadows.
- Vertical exaggeration: Apply modest vertical exaggeration (1–3×). Note exaggeration value in the figure caption.
6. Measurements and scale calibration
- Calibrate: Verify scan size and Z calibration against known standards.
- Add measurement overlays: Draw line profiles, roughness (Ra, RMS) boxes, or particle size annotations and export numeric values for methods.
7. Export settings for publication
- Resolution: Export at 300–600 DPI for raster images; for vector overlays export to SVG or EPS if supported.
- Format: Use TIFF for high-quality raster (16-bit if available). Use PNG for web, JPEG only if file size is necessary.
- Color space: Export in RGB; convert to CMYK later only if required by the journal during layout.
8. Figure assembly tips
- Consistency: Use consistent colormaps, scale bars, and font across all figures.
- Panels: Arrange multi-panel figures with aligned scales and shared colorbars when comparing data.
- Captions: Report processing steps, filters, scale, vertical exaggeration, and measurement methods in the caption.
9. Quick checklist before submission
- Data loaded and cropped
- Leveling and artifact correction applied
- Appropriate filtering (minimal)
- Perceptual colormap and scaled contrast
- Scale bar and color legend present
- Measurements calibrated and reported
- Export at journal-specified resolution/format
If you want, I can generate a short, publication-ready caption template and an export preset list tailored to a specific journal—tell me the journal or required DPI.
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